By John N. Abelson, Melvin I. Simon, M. Ian Phillips
Antisense expertise is the facility to control gene expression inside of mammalian cells supplying robust experimental methods for the research of gene functionality and gene law. for instance, tools which inhibit gene expression let stories probing the conventional functionality of a particular product inside a mobile. Such method can be utilized in lots of disciplines resembling pharmacology, oncology, genetics, mobilephone biology, developmental biology, molecular biology, biochemistry, and neurosciences. This quantity can be a very vital instrument in biomedically-oriented research.The severely acclaimed laboratory common for greater than 40 years, equipment in Enzymology is likely one of the such a lot hugely revered guides within the box of biochemistry. given that 1955, each one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless appropriate today-truly an important booklet for researchers in all fields of lifestyles sciences.
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Extra info for Antisense Technology, Part B
Sci. 23, 45 (1998). W. J. Zhu, J. F. Wang, S. Vicini, and D. R. Grayson, Mol. Pharmacol. 50, 23 (1996). 20 A. Nicot and D. W. Pfaff, J. Neurosci. Methods 71, 45 (1997). a Antisense to initiation codon of rat GABAA receptor Ͳ2 subunit mRNA Antisense to a nonoverlapping section of the Ͳ2 mRNA in the 3Ј direction from the target of antisense Antisense to a section of the 5Ј untranslated region of the Ͳ2 mRNA Complementary to antisense 1 Two central bases of antisense 1 interchanged Four central bases of antisense 1 interchanged Six ‘‘peripheral’’ changes in the sequence of antisense 1 Antisense to dopamine D2 receptor mRNA 1–4 4 4 1 2 1–4 4 4, 5 ANTISENSE RECEPTOR TARGETS Ͳ2 subunit antisense 1 2 Sequence Key to references: (1) J.
14  TARGETED GABAA RECEPTORS 27 mRNA is often used in in vivo antisense studies involving CNS proteins and has been successful in a number of studies targeting different neuronal proteins. The nucleotide sequences of antisense ODNs used to target the Ͳ2 subunit as well as control ODNs are shown in Table I. All experiments were carried out using fully phosphorothioate-modiﬁed ODNs. All sequences have been controlled for homologies to identiﬁed rodent gene sequences in the EMBL database. 18 Antisense ODNs may best be viewed as relatively speciﬁc, often offering a high degree of speciﬁcity.
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Antisense Technology, Part B by John N. Abelson, Melvin I. Simon, M. Ian Phillips