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Depart it to our specialists at QuickStudy to aid clarify the advanced international of biochemistry in an easy-to-understand style. This 3-panel (6-page) advisor offers the main complete details at the subject―definitions, formulation, molecular constitution, and full-color charts and illustrations highlighting vital chemical techniques.
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Extra info for BarCharts QuickStudy BioChemistry
When isolated from cells and deproteinized with phenol and chloroform (or proteases), the polymerase and other proteins are removed, allowing the nascent strands to collapse and bind to complementary sequences of the template. The RI/TI have multiple nascent strands and would be only partially double stranded and mostly single stranded; the RF/TF have only one or two nascent strands and would be almost or fully double stranded even without nuclease digestion. Finally, limited digestion of the deproteinized RNAs with RNase cleaves off all single-stranded RNA chains and converts these structures to fully double-stranded RF or TF molecules product is one of the six size classes of subgenomic mRNA.
The genome is associated with a helical nucleocapsid composed of the N protein of 50– 60 kDa. Surrounding the nucleocapsid is a membrane in which is embedded the envelope or spike (S) glycoprotein of 180 kDa, which may be cleaved in half by a host protease and which produces characteristic surface projections on the virions. Also embedded in the membrane are two other envelope proteins: a multi-spanning membrane (M) glycoprotein of 23 kDa essential for the assembly of virions and an E protein of 10 kDa, which although not absolutely essential for the formation of virions, is required for the release of large numbers of virions from infected cells (Kuo and Masters 2003).
J Gen Virol 75:3041–3046 van der Meer Y, Snijder EJ, Dobbe JC, Schleich S, Denison MR, Spaan WJ, Locker JK (1999) Localization of mouse hepatitis virus nonstructural proteins and RNA synthesis indicates a role for late endosomes in viral replication. J Virol 73:7641– 7657. van der Most RG, Bredenbeek PJ, Spaan WJM (1991) A domain at the 30 end of the polymerase gene is essential for encapsidation of coronavirus defective interfering RNAs. J Virol 65:3219–3226 van der Most RG, Luytjes W, Rutjes S, Spaan WJM (1995) Translation but not the encoded sequence is essential for the efficient propagation of the defective interfering RNAs of the coronavirus mouse hepatitis virus.
BarCharts QuickStudy BioChemistry by Inc. BarCharts