Biological Role of Indolealkylamine Derivates - Proceedings by Silvlo Garattini and Parkhurst A. Shore (Eds.) PDF

By Silvlo Garattini and Parkhurst A. Shore (Eds.)

ISBN-10: 0120329069

ISBN-13: 9780120329069

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Extra info for Biological Role of Indolealkylamine Derivates - Proceedings of a Symposium held at The College of Physicians and Surgeons, Columbia University, New York, New York, 10–12 May 1967

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1967). The mast cell enzyme was precipitated between 20 and 45% saturation with ammonium sulfate and assayed by the procedure of Sat0 et al. (1967). The activities are presented in mpmoles of tryptophan hydroxylated per milligram of protein per hour and represent the average of duplicate assays in a typical experiment. The use of mercaptoethanol to stabilize and measure hydroxylase activity was first reported by Nagatsu et al. (1964) for tyrosine hydroxylase. , 1967). This requirement has now been observed for tryptophan hydroxylase from all other mammalian tissues examined to date.

DEAE-celluloseeluate 54 6. Hydroxylapatite eluate 5 7. 1 37 Insoluble 60 Soluble 61 Soluble From 39 gm (dry weight) of purified mitochondria. 001 per minute at 250 mp using the spectrophotometric assay of Tabor et al. (1954) in whioh benzylamine is employed as substrate. clnsoluble means that detergent is required for solubility. Soluble means that no detergent is needed and that the enzyme precipitates instead of floats when ammonium sulfate is added to precipitate the enzyme. a b Properties of Purified Enzyme PURITYOF ENZYME The fact that the purified enzyme migrated as B single component during zone electrophoresis might be considered as evidence that it had reached a high stage of purity.

Proflavin 3. Methylene blue 4. Quinine Fin81 conc. 5 x 2 x 1x 10-5 10-5 10-4 10-5 10-4 10-4 9 24 39 27 18 12 a The standard benzylamine assay waa used. T h e inhibitor waa preincubated for 16 minutes with the enzyme (30 units, specific activity 8400) and then aaaayed. , 1966b), the studies will not be discussed here in detail. Discussion Biochemistry has advanced to such a sophisticated stage that enzyme purification per se is not considered exciting or challenging work. However, BEEF LIVER MITOCHONDRIAL MONOAMINE OXIDASE 57 our laboratory has virtually slaved over this problem of obtaining pure preparations of mitochondrial MA0 for the past 6 years.

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Biological Role of Indolealkylamine Derivates - Proceedings of a Symposium held at The College of Physicians and Surgeons, Columbia University, New York, New York, 10–12 May 1967 by Silvlo Garattini and Parkhurst A. Shore (Eds.)


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