By Russell C. Johnson
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Additional resources for Biology of Parasitic Spirochaetes
Walby. Cultivation of leptospires: Fatty acid requirements. Appl. Microbiol. 23^:1027-1028, 1972. Kadis, S. L. Pugh. Urea utilization by Leptospira. Infect. Immun. 10^793-801, 1974. Kelly, R. Cultivation of Borrelia hermsi. Science 172:443-444, 1971. B. W. Watson. Biologically active endotoxins from Salmonella mutants dificient in O- and R-polysaccharides and heptose. J. Bacteriol. 94: 1320-1326, 1967. C. M. Jenkin. Isolation of an unusual positional iomer of hexadecenoic acid from a parasitic leptospire.
They were, however, able to grow this organism in liquid medium made up of trypticase and soy extract (T. soy media). It is not known why the peptone yeast extract medium does not support growth of this organism. We have looked at base medium peptones in order to determine which one or ones were best for the cultivation of some of the tréponèmes. The number of days needed to form visible colonies on pre-reduced solid agar medium in rubber stoppered prescription bottles was used to determine the effectiveness of the various media and the rate of colony formation can be used as an indication of the nutritional proficiency of the medium.
Serotupe illini (3055), PTA negativelyLow magnification illustrating a typical long cell mensuration measurements. 0 μ^. , Borrelia recurrentis, the Reiter organism and a rumen Borrelia. 5 to 2% followed by OSO4 gives adequate preservation of leptospiral membranes but may not completely prevent nucleoplasmic condensation (Fig. 10). g. 4 to 6%, may severely damage the outer membrane. 5% 0s0 4 at RT; (2) 1- to 2-hr at RT in saturated aqueous uranyl acetate (ca 2%) after centrifuging the osmium prefixed cells; (3) recentrifugation from the uranyl acetate and suspension of the cells in warm agar (3-5%); (4) after solidification, the agar is minced and fixed overnight in 2% OSO4 at 4°; (5) dehydration with 50 to 100% ethanol; (6) infiltration and polymerization with divinylbenzene cross-linked methacrylates after the method of Kushida (12).
Biology of Parasitic Spirochaetes by Russell C. Johnson