By Jianhua Zhao, Haishan Zeng (auth.), Rongguang Liang (eds.)
This ebook presents an creation to layout of biomedical optical imaging applied sciences and their purposes. the most subject matters contain: fluorescence imaging, confocal imaging, micro-endoscope, polarization imaging, hyperspectral imaging, OCT imaging, multimodal imaging and spectroscopic platforms.
Each bankruptcy is written by means of the area leaders of the respective fields, and may hide:
- principles and boundaries of optical imaging technology,
- system layout and sensible implementation for one or particular functions, together with layout directions, method configuration, optical layout, part specifications and choice, method optimization and layout examples,
- recent advances and functions in biomedical researches and medical imaging.
This publication serves as a reference for college students and researchers in optics and biomedical engineering.
Read or Download Biomedical Optical Imaging Technologies: Design and Applications PDF
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Additional resources for Biomedical Optical Imaging Technologies: Design and Applications
6. 6 Spectroscopy System Calibration A spectrometer must be calibrated before usage. The calibration processes usually involve two distinct operations: wavelength calibration and spectral response calibration. For certain quantitative measurements, intensity calibration is also necessary. 1 Wavelength Calibration The wavelength calibration of a spectrometer is to correlate the wavelength to the position of the CCD pixel or the grating position in a monochromator. Wavelength calibration is usually done with a standard lamp.
The CCD has a 16-bit dynamic range and is liquid nitrogen-cooled to 120ıC. 2) matches the numerical aperture (NA D 0:22) of the fiber, so the throughput is much better than that of a traditional f /4 Czerny-Turner spectrographs . The spectral resolution of the system with 100-m fibers is 8 cm 1 . 6 Aberration Correction and Hardware Binning As discussed in Sect. 3, conventional spectrograph is equipped with a straight slit, and the image of the straight slit is parabolic through a plane grating.
M / IL . 8) where EL . / and IL . / are the emission spectra and measured spectra of current system of the standard lamp used for spectral response calibration. IR . / is the properties of the reference standard. IR0 . / and I 0 . / are the measured spectra of the reference standard and the sample. m is the wavelength at the maximum of the spectra of the reference standard. By inspection of Eq. 8, the physical meaning of the calibration procedure is straightforward. Intensity-calibrated spectrum is obtained by normalizing the wavelength and spectral response-calibrated sample spectra to the maximum of the wavelength and spectral calibrated spectrum of the reference standard.
Biomedical Optical Imaging Technologies: Design and Applications by Jianhua Zhao, Haishan Zeng (auth.), Rongguang Liang (eds.)