By Jonathan Y. Richmond, Robert W. McKinney
Describes the mixtures of ordinary & detailed microbiological practices, safeguard equip., & amenities that are instructed for paintings with quite a few infectious brokers in a variety of labs. This revision takes under consideration such matters as worldwide trouble approximately rising & reemerging infectious ailments; elevated curiosity in prion ailments with the id of BSE in England; the prevalence of numerous lab-associated infections; elevated problem concerning the nationwide & intl. move of infectious microorganisms; & growing to be issues approximately bioterrorism, & the elevated protection wishes of our microbiological labs.
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Extra resources for Biosafety in Microbiological and Biomedical Laboratories
The airflow in the s upp ly and e xha ust c om pon ents is monitored and the HVAC control system is designed to prevent sustained positive pressurization of the labor atory. The Clas s III ca binet shou ld be d irectly conne cted to the exhau st system . If the Class III cabinet is connected to the supply system, it is done in a manner that prevents positive pressurization of the cab inet. 15. The supply air to and exhaust air from the cabinet room, inner change room, and anteroom pass through HEPA filter(s).
The laboratory director has the final responsibility for assessing each circu ms tanc e and dete rm ining w ho m ay ent er or w ork in the labora tory or anim al room . 2. , im mu nizatio n) m ay ent er the labor atory. 3. A biohazard sign must be posted on the entrance to the laborator y when etiolo gic agen ts are in us e. Appro priate information to be posted includes the agent(s) in use, the biosafety level, the required immunizations, the investigator’s name and telephone number, any personal 22 Laboratory Biosafety Level Criteria – Biosafety Level 2 prote ctive e quipm ent th at m ust b e wo rn in th e labo rator y, and an y proced ures req uired for e xiting the labo ratory.
Vacuum lines are protected with liquid disinfectant traps and HEPA filters, or their equivalent. Filters must be repla ced as ne ede d. An altern ative is to us e por table vacuum pumps (also properly protected with traps and filters). 13. An eyewash station is readily available inside the laborator y. 14. Illumination is adequate for all activities, avoiding reflections and glare that could imped e vision. 15. The Biosafety Level 3 facility design and operational procedures must be documented.
Biosafety in Microbiological and Biomedical Laboratories by Jonathan Y. Richmond, Robert W. McKinney